p38α mapk signaling pathway  (MedChemExpress)

Journal: Immunobiology

Article Title: Giardia duodenalis triggered neutrophil extracellular traps in goats.

doi: 10.1016/j.imbio.2025.152894

Figure Lengend Snippet: Fig. 6. NETs release and ROS production was associated with NADPH oxidase, ERK1/2 and p38 MAPK signaling pathway (A) NETs quantitation. (B) ROS level. Goat neutrophils were pretreated with NADPH oxidase inhibitor DPI (10 μM), ERK1/2 inhibitor U0126 (50 μM), p38 MAPK signaling pathway inhibitor SB202190 (10 μM) respectively for 30 min, and stimulated with Giardia (ratio 1:1) for 2 h. Zymosan (1 mg/mL) was used as a positive control. Data were expressed as mean ± SD (n = 5). P < 0.05 was considered significant (*P < 0.05, ***P < 0.001).

Article Snippet: In inhibition tests, prior to stimulation with G. duodenalis (at a ratio of 1:1), neutrophils were pretreated with 10 μM of the NADPH oxidase inhibitor Diphenyleneiodonium chloride (DPI, Sigma-Aldrich, USA), 50 μM of ERK1/2 inhibitor U0126 (Sigma-Aldrich, USA), 10 μM of the p38 MAPK signaling pathway inhibitor SB202190 (Sigma-Aldrich, USA), 100 μM of TLR2 inhibitor C29 (MedChemExpress, USA) and 10 μM of TLR4 inhibitor TLR4-IN-C34 (C34, MedChemExpress, USA) respectively for 30 min 30 min. Zymosan was used as the positive control at a concentration of 1 mg/mL.

Techniques: Quantitation Assay, Positive Control

Journal: Biomolecules

Article Title: Anti-Inflammatory and Anti-Migratory Effects of Morin on Non-Small-Cell Lung Cancer Metastasis via Inhibition of NLRP3/MAPK Signaling Pathway

doi: 10.3390/biom15010103

Figure Lengend Snippet: The suppressive effects of morin on the MAPK signaling pathway (ERK/JNK/p38) in LPS+ATP-stimulated NSCLC cells: A549 ( A ) and H1299 ( B ) cells. NSCLC cells were treated with morin at doses ranging from 0 to 132 μM for 24 h. Following treatment, the cells were stimulated with LPS (1 mg/mL) for 6 h, followed by stimulation with ATP (5 nM) for an additional 30 min. Western blotting and band density measurements were used to analyze the effects of morin on the expression of MAPK signaling pathway components (ERK, JNK, and p38) in NSCLC cells. These data are presented as percentages relative to the LPS+ATP-stimulated group, which is defined as 100%. These results are reported as the mean ± standard deviation, with * p < 0.05, ** p < 0.01, and *** p < 0.001, compared to the control group (LPS+ATP-stimulated group). Original Western blot images can be found in .

Article Snippet: Primary antibodies for Western blot analysis to detect NLRP3, ASC, caspase-1, invasive proteins (u-PA, u-PAR, and MT1-MMP), EMT markers (fibronectin, N-cadherin, and vimentin), and MAPK signaling pathway (phospho-ERK, ERK, phospho-JNK, JNK, phospho-p38, and p38) protein expression, and the secondary antibodies, namely, anti-rabbit or anti-mouse IgG conjugated with horseradish peroxidase, were obtained from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Western Blot, Expressing, Standard Deviation, Control

Journal: Heliyon

Article Title: Monocytes and macrophages: Origin, homing, differentiation, and functionality during inflammation

doi: 10.1016/j.heliyon.2024.e29686

Figure Lengend Snippet: Overview of different Integrin expressions in various inflammatory conditions-.

Article Snippet: α 4 β 1 , • Liver ischemia-reperfusion injury (IRI) • Murine (Male Sprague Dawley rats)- Leukocytes (Neutrophils and macrophage) , • Fibronectin- α 4 β 1 interactions increases the expression of MMP9 and pro-inflammatory mediators by activating the p38 MAP kinase cell signaling pathway. , [ ] .

Techniques: Expressing, Protein-Protein interactions, Activation Assay, Mouse Assay, Inhibition